Whiff of the past from bat guano

In an experiment to understand better how ancient artefacts are altered by the sediment in which they are buried for thousands of years, Australian archaeological scientists buried bones, stones, charcoal and other items in bat guano, cooked it, and analysed how this affected the different items.

The researchers, PhD student Conor McAdams at University of Wollongong (UOW) and his supervisors, Distinguished Professor Richard Roberts (UOW, Director of the ARC Centre of Excellence for Australian Biodiversity and Heritage) and Associate Professor Mike Morley at Flinders University, are seeking to expand our knowledge of the first modern humans to arrive in Southeast Asia.

ARC Future Fellow Associate Professor Morley, who is leading a project to reconstruct ground conditions at various Late Pleistocene sites across Southeast Asia, says the latest article (DOI: 10.1016/j.jasrep.2021.102947) is an important step to develop and confirm accurate timelines of occupancy and abandonment of these ancient locations.

“Caves in tropical regions form a key resource for those reconstructing human evolution and dispersals,” he says. “However, the complex sedimentary records they contain remain under-interpreted because of the poorly constrained effects of humid tropical climates upon archaeological site formation processes.”

Part of the experiment conducted on the bat guano from Vietnam.

“Our bat poo experiment might seem like mad science, but it is helping to fill gaps in the story of the peopling of Southeast Asia over the last tens of thousands of years,” Mr McAdams explains.

The bat guano experiment was inspired during an archaeological dig at Con Moong Cave, one of the most significant archaeological sites in Vietnam, with a history of occupation that began more than 42,000 years ago.

While excavating a section of the cave floor they discovered a thick layer of ancient, soaking wet bat guano. Since caves once occupied by our human ancestors are often also occupied by bat colonies at different times, it is not uncommon for any archaeological artefacts they contain to be buried in guano-rich sediments.

In tropical regions, bats often live in much bigger colonies as there is generally more biomass. That means there is a tendency for bigger guano deposits to form. The bat guano in Con Moong Cave is almost 4 metres thick.

“We wanted to understand what the environment was like, thousands of years ago, in Con Moong Cave,” Mr McAdams says.

“We also wanted to understand how that environment might have changed, and whether archaeological remains might have been destroyed as a result of those changing environments, or the presence of bat guano.

“Bat guano is important when interpreting archaeological caves, because it is known to become acidic and destroy archaeological materials. But it also forms phosphate minerals, which can be useful as environmental indicators.”

Associate Professor Mike Morley, left, with Conor McKenna at Flinders University.

To better understand how the waterlogged guano in Con Moong Cave might have altered bones, stones, charcoal and other organic remains, the researchers designed a laboratory experiment.

Back in Australia, they collected “a couple of large Santa-sacks” of guano from a cave in New South Wales that is home to a large colony of bats with a similar insect diet to the bats of Con Moong Cave.

“We were very lucky to be taken to the largest known colony of horseshoe bats in Australia by some researchers who work for the Department of Primary Industries in NSW. Standing in that chamber, surrounded by thousands of tiny, shrieking bats was a truly intense experience. I have goosebumps thinking about it,” he says.

At the University of Wollongong, the researcher group placed a range of materials similar to those likely to have been left behind by hunter-gatherers in Southeast Asia, including bone, bamboo, charcoal, limestone and clay, in 24 containers and covered them in a thick layer of wet bat guano. To recreate the hot, humid conditions experienced in Con Moong Cave tens of thousands of years ago, they put the containers in an oven set to 30℃.

“We found that all the materials were subject to surprisingly rapid changes, especially bone and limestone,” says Mr McAdams, pictured at work with Associate Professor Morley at Flinders University Archaeology.

“We could observe these materials changing chemically from month-to-month, and the techniques we used, such as thin-section analysis and scanning electron microscopy, helped us to understand how and why this was happening.

“Our results told us that the cave was probably very wet when the guano was deposited and that the guano might not have become acidic, due to the waterlogged and anoxic environment that formed.

“Despite this, we found that any archaeological materials buried under such conditions were very unlikely to survive. These results underscore the destructive potential of guano in archaeological sites, even where environmental conditions prevent it from becoming acidic.

“This helps us to understand environmental conditions in the past and interpret the distribution of archaeological materials in caves. We have a lot more work to do, but our results suggest bacteria play a prominent role in the destruction of archaeological materials under these conditions.”

This research was funded by the Australian Research Council, through an Australian Laureate Fellowship (FL130100116) to Bert Roberts, and by UOW through a Postgraduate Award and an International Postgraduate Research Scholarship to Conor McAdams. Mike Morley is supported by an ARC Future Fellowship award (FT180100309).

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